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. Author manuscript; available in PMC: 2020 Nov 1.

Published in final edited form as: Cytokine. 2019 Jul 25;123:154789. doi: 10.1016/j.cyto.2019.154789

Figure 1. — A) Using RNA-sequencing data [24], we determined average CCL20 transcript abundance in NAFLD samples across a spectrum of liver histology (36 normal, 50 steatosis, 52 inflammation, and 53 fibrosis). The ordinal logistic regression showed a significant positive trend (β = 0.244; P = 4.8E-4), whereas the pairwise comparisons conducted with logistic regression showed a significant difference only between normal and fibrosis histological grades (P_adj =1.3E-03). B) CCL20 gene expression was measured in a subset of NAFLD patients with steatosis (n=10), lobular inflammation (n=10), advanced fibrosis (n=10), and normal liver histology (n=10). Real time qPCR was performed using Taqman gene expression assays for CCL20 and GAPDH. The Kruskal-Wallis one-way analysis of variance test with a Dunn post-hoc test were used to assess differences in expression between each histological grade of NAFLD and normal histology. *P≤0.05, **P≤0.001, and *** P≤ 0.0001.

Figure 1. — A) Using RNA-sequencing data [24], we determined average CCL20 transcript abundance in NAFLD samples across a spectrum of liver histology (36 normal, 50 steatosis, 52 inflammation, and 53 fibrosis). The ordinal logistic regression showed a significant positive trend (β = 0.244; P = 4.8E-4), whereas the pairwise comparisons conducted with logistic regression showed a significant difference only between normal and fibrosis histological grades (P_adj =1.3E-03). B) CCL20 gene expression was measured in a subset of NAFLD patients with steatosis (n=10), lobular inflammation (n=10), advanced fibrosis (n=10), and normal liver histology (n=10). Real time qPCR was performed using Taqman gene expression assays for CCL20 and GAPDH. The Kruskal-Wallis one-way analysis of variance test with a Dunn post-hoc test were used to assess differences in expression between each histological grade of NAFLD and normal histology. *P≤0.05, **P≤0.001, and *** P≤ 0.0001.