Figure 5. miR-590-5p expression in LX-2 cells.
A) miR-590–5 expression levels were measured in activated (gray bar) LX-2 cells and cells grown on Matrigel (black bar). B) LX-2 cells were treated with 250 ng/mL CCL20 (CCL20: gray bar) or untreated (NTC: black bar) for six hours. Data were normalized using RNU48 and RNU6B. The −ΔΔCt method was used to determine fold-change of miR-590-5p expression. All experiments were performed in triplicate. *P<0.05.