Fig. 1.

Replication kinetics of PEDV KD & AA. Confluent Vero cells were inoculated with PEDV KD (A) or AA (B) at an MOI of 5 and incubated at 37 °C for 1 h. After thorough washing with PBS, fresh MEM containing (A) Mock-medium, TPCK-treated trypsin (1 μg/ml) or TPCK-treated trypsin (1 μg/ml)+inhibitor or (B) elastase (1 μg/ml), elastase (1 μg/ml)+inhibitor were added to the virus infected Vero cells. Viral RNA was extracted from the cells at 0, 2, 4, 6, 12 or 24 h post-inoculation (PI) for real-time qRT-PCR, and genome copy numbers were calculated by plotting Ct values against a standard curve gene-rated using a series of dilutions of in-vitro transcribed PEDV RNA genome. Error bars show standard deviations, and asterisks indicate significant difference (p < 0.05) in virus RNA titiers, compared to those at 0 h.