Infusion of sPRR impaired autonomous nervous system in obese male mice. (A) Mean arterial pressure response (ΔMAP) to chlorisondamine injection (1mg/kg body weight, i.p) represents the difference in MAP one hour before and one hour after drug injection in HF-fed C57BL/6 male mice infused with vehicle (Veh, n=5) or sPRR (sPRR, n=6). (B) Heart rate response (ΔHR) to propranolol injection (5mg/kg body weight, i.p) represents the difference in HR one hour before and one hour after drug injection. (C) HR response (ΔHR) to atropine injection (5mg/kg body weight, i.p) represents the difference in HR one hour before and one hour after drug injection. Values are mean±SEM. Differences between groups were revealed using One–way ANOVA followed by post hoc Holm-Sidak multiple comparison. *P<0.05 compared with Veh.