Fig. 5.

Ly6C^hi monocytes, NKT, and NK cells in temporal gating of IAV infection. The left lung lobe was digested, dissociated, and analyzed by flow cytometry. a CD45⁺ cells (as a % of live) and CD45+ cell numbers. Two-way ANOVA, p < 0.01 for time of infection <0.05 for day of dissection and p < 0.05 for interaction. For p < 0.05 for time of dissection, time of infection and interaction. b Macrophages, two subsets of dendritic cells (CD11b + and CD103+) using gating strategies from Supplementary Fig. 10a. c Ly6C^hi inflammatory monocytes with images from representative experiment. For monocytes, two-way ANOVA, p < 0.0001 for time of infection, p < 0.001 for day of dissection and N.S. for interaction. d Neutrophils (top panel) and absolute NK1.1⁺ cells (middle panel), and % of NK1.1⁺ cells (bottom panel). Two-way ANOVA. For % NK1.1⁺ cells, p < 0.05 for time of infection, <0.05 for day of dissection, and p < 0.05 for interaction. For neutrophils % and NK cell numbers, no comparisons were significant. For a–d, representative results from one experiment are shown. Experiments were repeated with similar results three times. e NK cells and Ly6C^hi cells (as % of total CD45 cells) from Bmal1^fl/flERcre⁺ mice and their cre⁻ littermates. For NK cells two-way ANOVA, p = ns for time of infection, and p < 0.05 for day of time of dissection in Cre⁻ animals, but no difference in Cre⁺ groups and p < 0.05 for interaction. The experiment was done once. c–e Using gating strategies from Supplementary Fig. 10b. The data expressed as mean ± SEM. Source data are provided as a Source Data file