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. 2019 Sep 11;10(9):670. doi: 10.1038/s41419-019-1873-x

Fig. 1. TGF-β1-induced miR-133a blocks pulmonary fibroblast differentiation.

Fig. 1

a RT-PCR analysis of TGF-β1-induced upregulation of miR-133, -1, -143, -145 and -21 in human lung fibroblast (HFL) cells. Cells were transfected with 30 nM of various miRNAs (b), miR-133a mimic (c), miR-133a inhibitor (d) or the control mimic (CTL) for 24 h, and then were stimulated without or with 1 ng/mL TGF-β1 for 48 h. α-SMA levels were analyzed by western blot and GAPDH was used as an internal control. Experiments were performed three times with similar results. e HFL cell differentiation was assessed by immunofluorescence staining for α-SMA. Up: representative staining images of α-SMA–positive stress fibers (green) and DAPI (blue) showing nuclei under confocal laser scanning microscopy. Down: bright field image and DAPI staining of nuclei (blue) (scale bar = 30 μm)