Fig. 4.

Absence of IRF3 phosphorylation in Spont-PyMT tumors after DMXAA injection. a Spont-PyMT mice and Trans-PyMT mice received one i.p. injection of DMXAA. Three hours later, tumors were collected and tumors slices were stained with anti-EpCAM (blue) and anti-pIRF3 (red) mAbs. Representative images from Trans-PyMT tumors (left) and Spont-PyMT tumors (right) are shown. b In Trans-PyMT tumors, some pIRF3⁺ cells (red) are myeloid (F4/80⁺, green), or endothelial cells (CD31⁺, yellow). Scale bars = 50 µm. The data are representative of three Spont-PyMT and five Trans-PyMT mice, from five independent experiments