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. 2019 Sep 11;9:13084. doi: 10.1038/s41598-019-49234-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Genome-wide screening for human SA-binding proteins (SABPs). (A) Schematic depiction of screening for SABPs in HEK293 cell lysates using the photo-reactive SA analog 4AzSA (see Materials and Methods for more details). (B) Venn Diagram summarizing the overlap among proteins identified by the 4AzSA screening approach in three replicate samples (#1, #2 and #3) that were either subjected to UV crosslinking (+UV) or not (−UV). A total of 2,604 proteins were identified in the three samples exposed to UV irradiation, with 2,244 proteins found in all three replicates. In the absence of UV exposure, a total of 1168 proteins were identified, with 375 found in all three replicates. (C) Heat map of protein abundance in the three replicates (#1, #2 and #3) performed with (3 left lanes) or without (3 right lanes) UV exposure (+UV and −UV). (D) EdgeR scatter plot showing the quantitative enrichment of 2,329 4AzSA crosslinked, immuno-selected proteins as compared to their levels in the published HEK392 quantitative proteomic database. Differentially enriched proteins (with false discovery rate (FDR) < 0.05 and enrichment ratio >5) are shown in red.