Fig. 5.

Upregulation of VEGFB was dependent on circAnks1a expression following SNL. a Colocalization of circAnks1a and VEGFB. Scale bar, 50 μm (n = 3). b, c Intrathecal injection of circAnks1a siRNA decreased the mRNA and protein expression of VEGFB in the spinal dorsal horn of rats on day 14 following SNL (^*P < 0.05, ^**P < 0.01 vs. sham group, ^#P < 0.05, ^##P < 0.01 vs. the corresponding scramble group, two-tailed one-way ANOVA, n = 5 for (b) and n = 4 for (c)). d, e Twenty-one days following intraspinal injection of recombinant AAV-circAnks1a-EGFP, the expression of VEGFB was significantly upregulated at mRNA and protein levels (^*P < 0.05, ^**P < 0.01 vs. the AAV-EGFP group, two-tailed one-way ANOVA, n = 5 for (d) and n = 4 for (e)). The data are presented as the mean ± s.e.m. Source data are available as a Source Data file