Figure 2.

Antibacterial activities of AgNP, AgNP@LA, and Ag-ND@BSA. (A) Antimicrobial tests of non-oxidized AgNP, AgNP, and AgNP@LA against E. coli. (B) Aggregation and precipitation of three different types of particles in LB broth deposited on microplates. The concentration of these particles decreases from 500 µg mL⁻¹ to 62.5 µg mL⁻¹ (1st to 4th row at each time point). The particles in the first column of each sample were dispersed in distilled deionized water (DDW), serving as the negative control. The side view shows the precipitation of AgNP and AgNP@LA at 500 µg mL⁻¹ after the sample preparation for 24 h. (C) Concentration-dependent measurements for the antibacterial activities of AgNP@LA and Ag-ND@BSA at 50, 250, and 500 µg mL⁻¹. (D) MIC measurements of AgNP@LA and Ag-ND@BSA. The measurements were conducted with a spectrophotometer for the O.D. of the E. coli suspensions at 600 nm.