Table 2.
Oligonucleotides
| Primer | Purpose | Sequence 5′ to 3′ |
|---|---|---|
| lrs1-1 | Overexpression construct | TTAAAGAGGAGAAATTAAGCGCCATCTCATGGGTTCGG |
| lrs1-2 | Overexpression construct | AGGAATTCCTCGAGAAGCTTCGCTGGGCGAAGCGCGAT |
| lrs1-3 | Overexpression construct | AAGCTTCTCGAGGAATTCCTG |
| lrs1-4 | Overexpression construct | CTAGAATTAAAGAGGAGAAATTAAGC |
| pqsAE-1 | Deletion construct | GATACAAAGCTTGCAAA GGCAGGCGAGACG |
| pqsAE-2 | Deletion construct | TCAGTCCAGAGGCAGCGCCTCGGTCAGGTTGGC CAATG |
| pqsAE-3 | Deletion construct | CATTGGCCAACC GACCGAGGCGCTGCCTCTGGACTGA |
| pqsAE-4 | Deletion construct | GATACAAAGCTTGCCTGCGACTCTTCCTGG |
| pqsAE-5 | Deletion construct | CCCAGTGTACTACGCAATGG |
| pqsAE-6 | Deletion construct | CCATGCAGGCGTCGCCAC |
| lasR_F | qRT-PCR | CGTACTGCCGATTTTCTGGG |
| lasR_R | qRT-PCR | AGTGCGTAGTCCTTGAGCAT |
| lasI_F | qRT-PCR | CTTTTCCGACTGTACGCTGG |
| lasI_F | qRT-PCR | AAAGCGCGATCTGGGTCTTG |
| rhlR_F | qRT-PCR | CCTGGAAAAGGAAGTGCGGC |
| rhlR_R | qRT-PCR | CCAGCCAGGCCTTGGGATAG |
| pqsA_F | qRT-PCR | GATATCGCTGCCGACTGCCA |
| pqsA_R | qRT-PCR | GGTGGAACCCGAGGTGTATT |
| 5s_F | qRT-PCR | GAACCACCTGATCCCTTCCC |
| 5s_R | qRT-PCR | TAGGAGCTTGACGATGACCT |
| lasI-cherry-1 | Reporter construct | GAT ACA AAG CTT CCG GGT TCA CCG AAA TCT ATC TC |
| lasI-cherry-2 | Reporter construct | CCT CGC CCT TGC TCA CCA TCT TCA CTT CCT CCA AAT AGG AAG C |
| lasI-cherry-3 | Reporter construct | GCT TCC TAT TTG GAG GAA GTG AAG ATG GTG AGC AAG GGC GAG G |
| lasI-cherry-4 | Reporter construct | GAT ACA AAG CTT CTA CTT GTA CAG CTC GTC CAT GCC |
| lasRI-5 | Complementation construct | GAT ACA AAG CTT CCA TGG GAC GCC TGT TCG |
| lasRI-3 | Complementation construct | GAT ACA AAG CTT TCT GTG TCG CCG AAC TGG |