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. 2019 Sep 11;9:13090. doi: 10.1038/s41598-019-49482-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of PrP^C in the human trabecular meshwork. (a) Immunoreaction of human TM section with PrP-specific antibody 3F4 followed by Alexa fluor 546-conjugated secondary antibody shows strong reactivity in all layers of the TM (panel 1). High magnification image demonstrates expression of PrP^C on the plasma membrane of TM cells (panel 2). A serial section reacted with mouse IgG and Alexa fluor 546-conjugated secondary antibody shows no reaction (panel 3). H&E staining of a serial section confirms the TM region and Schlemm’s canal (SC) (panel 4). Scale bar: 25 µm. (b) Non-permeabilized primary human TM cells reacted with 3F4 followed by Alexa Fluor 488-conjugated secondary antibody show expression of PrP^C on the plasma membrane (panel 1). No reaction is detected in control cells exposed to mouse IgG followed by the same secondary antibody (panel 2). Scale bar: 25 µm.