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. 2019 Sep 11;10(9):664. doi: 10.1038/s41419-019-1900-y

Fig. 6. Transplantation of murine macrophages secreting IL-4 (M-IL-4) attenuates lung inflammation in mice.

Fig. 6

Mice were exposed to LPS (15 mg/kg). After 0.5 h, PBS, M-IL-4 (2.5 × 105 cells/mouse), or M-Con (2.5 × 105 cells/mouse) was intratracheally transplanted (n = 8 for each group). The lungs were isolated at the indicated time points (n = 8 for each group). a The levels of TNF-α, IL-6, IL-1β in BALF at 6 h after LPS injection was determined by ELISA. b, c The levels of pro-inflammatory factors (TNF-α; IL-6; IL-1β) in lung homogenate at 6 h, 24 h and 48 h after LPS injection were determined by ELISA. d Total protein in BALF was determined by BCA protein assay (n = 6 for each group). e Mice were exposed to LPS (20 mg/kg). After 0.5 h, PBS, M-IL-4 (2.5 × 105 cells/mouse), or M-Con (2.5 × 105 cells/mouse) was intratracheally transplanted (n = 8 for each group), and Kaplan–Meier survival curves for mice are presented. *P < 0.05; **P < 0.01, the error bars represent ±SD