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. 2019 Sep 11;9:13167. doi: 10.1038/s41598-019-49755-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GN6-ELAA for Gram-negative bacterial OMVs. (a) Scheme of GN6 ELAA for bacterial OMVs. (b) Three Gram-negative bacterial OMVs showed highly sensitive binding to GN6 aptamer (●) rather than N40 control (○) at 250 nM concentration. (c) GN6 ELAA towards both Gram-negative and Gram-positive bacterial OMVs. (d) GN6 ELAA showed higher specificity to 5 μg/mL of Gram-negative bacterial OMVs compared to 5 μg/mL of Gram-positive bacterial OMVs from L. grayi and B. megaterium. Data represented mean ± SD values of three independent experiments.