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Validation of RT-qPCR assay parameters for primers targeting aiiO, gyrB, rho and rpoD.
| Parametera | aiiO b | gyrB | rho | rpoD |
|---|---|---|---|---|
| Efficiency (%) | 94.9 | 95.9 | 93.5 | 89 |
| R2 | 0.999 | 1 | 1 | 0.999 |
| Slope | −3.451 | −3.425 | −3.487 | −3.618 |
aEfficiency – calculated primer efficiency; R2 – correlation between log Cq and Cq in the standard curve; Slope – slope of the standard curve.
bPrimers used for PCR amplification: aiiO: F_aiiO_OP7/R_aiiO_OP7; gyrB: F_ gyrB_OP7/R_ gyrB_OP7; rho: F_ rho_OP7/R_ rho_OP7 (Table S1).
