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. 2019 Sep 11;10:4108. doi: 10.1038/s41467-019-12124-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 8 — Overexpression of COX7RP promotes oxygen consumption after hypoxia-mimetic treatment. a–c CI-mediated respiration. COX7RP-MCF7 and vector-MCF7 cells were incubated overnight in normoxic or hypoxic (1% O₂) conditions. The medium was then replaced with serum-free XF medium containing glucose and sodium pyruvate as substrates. Mitochondrial stress test was performed at 37 °C on a Seahorse Bioscience XF Extracellular Flux Analyzer using a Cell Mito Stress Test Kit and oxygen consumption rates (OCRs) were plotted a. Maximal OCRs b and basal extracellular acidification rate (ECAR) c are presented as means ± SD (n = 3 biologically independent samples). d, e CII-mediated respiration. Mitochondrial stress test was performed as described above using succinate as a substrate in the presence of rotenone d. OCRs are presented as means ± SD (n = 3 biologically independent samples). Maximal OCRs e were measured as the oxygen consumption rate after FCCP addition minus the non-mitochondrial respiration. *P < 0.05; **P < 0.01, two-way analysis of variance. Source data are provided as a Source Data file