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. 2019 Sep 11;9:13121. doi: 10.1038/s41598-019-49483-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Localization of lncRNA Neat2 and mRNAs coding for Polr2a and Ank2.3 in NE and NEBD oocytes. Single Z-scan confocal images from RNA FISH for (A) Neat2 RNA (B) Polr2a mRNA and (C) Ank2.3 mRNA in NE (0 h) and NEBD (nuclear envelope breakdown; 3 h) oocytes. RNA in grey and red and DNA in blue (DAPI). The cortex of the cell is depicted by a black or white dotted line. Representative images from at least three independent experiments (n ≥ 18), scale bar = 20 μm. Bacterial DapB RNA (Bacillus subtilis, str. SMY; EF191515.1) was used as a negative control. See also Suppl. Fig. 1C,D.