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. 2019 Sep 11;9:13121. doi: 10.1038/s41598-019-49483-5

Figure 4.

Figure 4

Spindle area contains assembled ribosomes. (A) Proximity ligation assay (PLA) detecting ribosome assembly using RPL24 and RPS6 markers (grey and green dots). EDTA was used for ribosome disruption. As a negative control (NTC) RPS6 antibody was omitted. The cortex of the oocytes is depicted by a black or white dotted line; dashed line shows spindle area. Representative images of three independent experiments (n ≥ 15); scale bar = 20 μm. (B) Quantification of ribosome assembly events in NEBD oocytes. Mean; error bars are SD; Student’s t-test, *P < 0.05; ***P < 0.001.