Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Sep 11;9:13121. doi: 10.1038/s41598-019-49483-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

ANK2 is translated at the newly forming spindle through cap-dependent translation. (A) Ribopuromycilation combined with Proximity ligation assay (Puro-PLA) were performed on NEBD stage oocytes in the absence or presence of cycloheximide (CHX) and eIF4E/eIF4G1 interaction inhibitor (4EGI-1). Microinjection of morpholinos (MO) was used to specifically suppress Ank2.3 mRNA translation in the oocyte. ANK2 translational events in grey and green scales, DNA in blue. The cortex of the cell is depicted by a black or white dotted line; dashed line shows spindle area. Representative images from at least three independent experiments (n ≥ 15), scale bar = 20 μm. As a negative control (NTC) ANK2 antibody was omitted. (B) Quantification of ANK2 translational events at the spindle area (black) and cytoplasm (grey). Mean, error bars are SD; Student’s t-test; *P < 0.05; **P < 0.01; ***P < 0.001; NS non-significant. See also Fig. 6D and Suppl. Figs 3 and 5.