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. 2019 Sep 11;9:13121. doi: 10.1038/s41598-019-49483-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Downregulation of ANK2 results in defects in oocyte meiosis. (A) Experimental scheme of downregulation of ANK2 in the oocyte. (B) Expression of ANK2 protein is decreased after microinjection of morpholinos (MO) against Ank2.3 mRNA. GAPDH was used as a loading control. Representative image from three independent experiments. (C) Morphology of the meiotic progression of oocytes microinjected with Control and Ank2.3 morpholinos (MO). RNA coding for H2B:GFP was co-injected with morpholinos to track chromosomes. Asterisk depicts polar body, scale bar 30 µm. (D) Quantification of abnormal phenotypes (aberrant spindle formation and cytokinesis – polar body extrusion) in the morpholino microinjected oocytes. From three independent biological experiments (n ≥ 31). Presented as mean ± SD, Student’s t-test **p < 0.01, NS non-significant. See also Suppl. Fig. 5.