Fig. 5. SREBP-1 knockdown synergizes Sorafenib’s effect on HCC s.c. tumor growth.

a MHCC97-H s.c. tumors were established by using MHCC97-H cells transfected with control or SREBP-1 siRNAs, or SREBP-1 siRNAs plus SREBP-1^mut expressing vector. MHCC97-H s.c. tumor-bearing mice received vehicle control, or Sorafenib at the IC₅₀ concentration, or 2 mg/kg Betulin or Sorafenib + Betulin orally every other day 10 times. At day 21 post treatment, mice were killed and tumors were obtained (N = 10). b Quantitative results of tumor volume and tumor weight from a. c Western blotting of SREBP-1 and apoptosis and proliferation-associated proteins in tumors from a. β-actin was used as internal control