Table 1.
Primer combinations used in PCR and qPCR.
| Code | Primer sequence (5′-3′) | Amplicon length (bp) | AT (°C) | Efficiency* | R2 |
|---|---|---|---|---|---|
| Ss_qGAO | F: CGAAAACGGCAACACCACCATT | 162 | 60 | 92.3 | 0.998 |
| R: GCTCGCACCATTGGGAAGTTTC | |||||
| Ss_qCHS | F: GCCGACTACCAGCTCACCAAACTC | 193 | 60 | 87.2 | 0,999 |
| R: CCTCATTAGGGCCACGGAACG | |||||
| Ss_qHQT | F: GCTACCTTGGGAACGTGGTCTTTACA | 194 | 60 | 91.2 | 0.997 |
| R: GTAACTGGGTCCACGGATTAGAGCC | |||||
| Ha_qACT | F: GCCGTGCTTTCTCTTTATGCCAGCGACC | 137 | 60 | 95.0 | 0.998 |
| R: AGCGAGATCAAGACGAAG | |||||
| Ha_qEF | F: ACCAAATCAATGAGCCCAAGAGACCCA | 131 | 60 | 99.6 | 0.987 |
| R: TACCGGGCTTGATCACACCAG | |||||
| Ss_GAO | F: CARTCGAATGGGCGATTTC | 532 | 54 | — | — |
| R: CTTTGAACCGTGGCTCCAA | |||||
| Ss_CHS | F: AAGCCATYAAAGAATGGGGAAA | 729 | 54 | — | — |
| R: CCRCCGACTTCTTCCTCATCTC | |||||
| Ss_HQT | F: GATATGGCTCGCGGGTTCTC | 617 | 56 | — | — |
| R: GCATCATGTACTGGTAGCCTGGTC |
*Determined only for qPCR primers; AT: annealing temperature.