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. 2019 Sep 11;10:4129. doi: 10.1038/s41467-019-12027-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Engineered translator cells produce AI-1 in response to AI-2. a Translator cells couple the AI-2 and AI-1 quorum sensing circuits using a dual plasmid system. Phosphorylated AI-2 activates expression of T7 RNA polymerase and subsequent expression of LasI which synthesizes AI-1. b AI-1 produced by CT104 pCT6 pLasI or PH04 pCT6 pLasI grown in different media with and without AI-2 addition. AI-2 was added as indicated at ~OD 0.1 and samples for AI-1 measurement were taken 6 h later. Error bars represent s.d. between technical duplicates. Source data are provided as a Source Data file