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. 2019 Sep 11;9:13147. doi: 10.1038/s41598-019-48299-7

Figure 1.

Figure 1

Activity of Actinoallomurus A8 culture supernatant fraction “<100 kDa” containing the wild Endopep-40. (A) Activity toward the substrate sucAlaAlaProPhe-AMC at various pH values. X axis: pH value, Y axis: relative activity (%). (B) Gliadin digestion by the wild E40 containing supernatant fraction after 2 hour of incubation at 37 °C, pH 3. Gel stained with Coomassie R250 brilliant blue. Lane 1: gliadin alone, lane 2: gliadin + E40 (<100 kDa fraction).