Fig. 3.

a Side scatter vs FL1 (GFP) dot plots obtained in one representative XY DFs cell line untransduced or transduced with GFP-miR548am-5p in the presence or absence of AZT. Numbers indicate the percentage of GFP-positive cells. b Cytofluorimetric histograms of Bax and Bcl-2 expression level in GFP-negative and -positive cells obtained in one representative XY DFs cell line after transduction with GFP-miR548am-5p. Numbers indicate the median fluorescence intensity. Bar graph on the right shows the ratio Bax/Bcl-2 of four XY DFs cell lines. *p < 0.01 between GFP-positive and GFP-negative cell populations. c Flow cytometry histograms of apoptosis evaluation in GFP-positive or -negative cell populations of one representative XY DFs cell line after transduction with GFP-miR548am-5p treated or not with CHX+TNF-α. Numbers represent the percentages of annexin V-positive cells. Bar graph showing flow cytometry analysis after cell staining with Annexin V-APC performed in triplicate and reported as means ± SD of four XY DFs cell lines