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. 2019 Sep 11;10:4113. doi: 10.1038/s41467-019-12068-x

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Endocardial cell sizes are not altered upon atrial chamber expansion. a–d Reconstructions of confocal z-stacks of representative hearts at 52 hpf of (a) WT, (b) upon Wnt8a overexpression, (c) WT, or (d) in nkx2.5^vu179 mutants. Endocardial GFP expression of Tg(kdrl:EGFP)^s843 or Tg(fli1a:nEGFP)^y7 is inverted in black/white. Endocardial inter-nuclear distances are indicated by arrows and a dotted line indicates the outline of the endocardium. Scale bars, 30 µm. e Quantifications of endocardial inter-nuclear distances reveal no significant differences between WT (kdrl:GFP n = 6 hearts, fli1a:nEGFP n = 9 hearts), upon Wnt8a overexpression (n = 6 hearts), or in nkx2.5^vu179 mutants (n = 9 hearts). Each dot represents one heart with an average of at least 20 length measurements within the endocardial atrium. f Quantifications of the atrial endocardial surface area reveals a significant increase upon Wnt8a overexpression (n = 7 hearts) or loss of Nkx2.5 (n = 10 hearts) compared to WT hearts (kdrl:GFP n = 12 hearts, fli1a:nEGFP n = 12 hearts). g Quantifications of the ratio of endocardial cell numbers relative to total atrial chamber area indicates no significant differences between WT (kdrl:GFP n = 11 hearts, fli1a:nEGFP n = 12 hearts), upon Wnt8a overexpression (n = 8 hearts), or in nkx2.5^vu179 mutants (n = 10 hearts). e–g Mean values ± SD are shown. One-way ANOVA was used to compare each condition with its WT control (ns: not significant, ****p < 0.0001)