Table 2.
Data Collection and Refinement Statistics
| Data Collection | RppC | RppC-TerS1–98 |
|---|---|---|
| Beamline | DLS I04 | ESRF ID30B |
| Wavelength (Å) | 0.9795 | 0.9762 |
| Space group | P43212 | C2221 |
| Cell dimensions (Å) | a = 56.88, b = 56.88 | a = 60.52, b = 101 |
| c = 132.27 | c = 82.63 | |
| α = β = γ = 90 | α = β = γ = 90 | |
| Resolution (Å) | 43.12–2.42 (2.54–2.4)a | 50.50–3.00 (3.18–3.00) |
| Total reflections | 46,007 (2,558) | 18,007 (3,017) |
| Unique reflections | 7,721 (389) | 5,163 (832) |
| Completeness (%) | 86.38 (78) | 97.7 (98.7) |
| Multiplicity | 6.0 (6.6) | 3.5 (3.6) |
| Mean I/(σI) | 13.6 (1.2) | 11.1 (6.7) |
| Rmerge | 0.073 (1.475) | 0.064 (0.105) |
| Rpim | 0.033 (0.619) | 0.041 (0.066) |
| CC 1/2 | 0.999 (0.584) | 0.992 (0.992) |
| Refinement | ||
| Rwork | 0.237 | 0.3124 |
| Rfree | 0.267 | 0.3321 |
| Number of atoms | 1,060 | 1,615 |
| Protein | 1,060 | 1,595 |
| Water | – | 20 |
| RMSD, bonds (Å) | 0.003 | 0.016 |
| RMSD, angles (°) | 0.679 | 1.75 |
| Ramachandran Plot | ||
| Preferred (%) | 95 | 86 |
| Allowed (%) | 5 | 14 |
| Outliers (%) | 1 | 0 |
a
Number in parentheses indicates values for the highest-resolution cell.