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. 2019 Aug 7;14:313–322. doi: 10.1016/j.omto.2019.08.001

Figure 4.

Figure 4

Exosome Derived from NSCLC Cells Promoted Angiogenesis

(A) Representative electron micrographs of exosomes of the typical size isolated from BEAS-2B cells conditioned and A549 cells conditioned medium exhibiting exosomal morphological traits. Scale bars, 100 nm. (B) Histogram showing the particle diameter (nm) of the small vesicles harvested from the medium of the A549 cells using the standard exosome isolation protocol. The amount of exosomes released into the conditioned media was normalized to the number of cells in the culture for conditioned media collection. (C) Western blotting revealed substantial expression of TSG101 and CD63 in exosomes isolated from the medium of BEAS-2B cells (BEAS-2B exo) and A549 cells (A549 exo). (D) The mRNA levels of proangiogenic factors VEGFA and Ang1 in HUVECs were determined by qRT-PCR. HUVECs were exposed to BEAS-2B cell-derived exosomes (+BEAS-2B exo), A549 cell-derived exosomes (+A549 exo), or alone (blank) for 48 h. (E) The protein levels of proangiogenic factors VEGFA and Ang1 in conditioned media of HUVECs were determined by ELISA. HUVECs were exposed to BEAS-2B cell-derived exosomes (+BEAS-2B exo), A549 cell-derived exosomes (+A549 exo), or alone (blank) for 48 h. (F) Representative images show the tube formation of HUVECs after the same exposure of corresponding exosomes. *p < 0.05; **p < 0.01.