Figure 6.

Exosomal LRG1-Mediated Promotion of Angiogenesis Was Dependent on TGF-β Signal Pathway

(A and B) The expression levels of TGF-β in HUVECs were determined by qRT-PCR (A) and western blotting (B). HUVECs were exposed to BEAS-2B cell-derived exosomes (+BEAS-2B exo) or A549 cell-derived exosomes (+A549 exo) or A549 cell-derived exosomes plus TGF-β inhibitor SM-16 (10 μg/mL; +A549 exo+SM-16). (C) TMLC assay for active TGF-β from conditioned media of HUVECs following the same exposure. (D) The mRNA levels of proangiogenic factors VEGF and Ang1 in HUVECs were determined by qRT-PCR. HUVECs were exposed to BEAS-2B cell-derived exosomes (+BEAS-2B exo), A549 cell-derived exosomes (+A549 exo), or A549 cell-derived exosomes plus TGF-β inhibitor SM-16 (+A549 exo+SM-16). (E) The protein levels of proangiogenic factors VEGFA and Ang1 in conditioned media of HUVECs were determined by ELISA. HUVECs were exposed to BEAS-2B cell-derived exosomes (+BEAS-2B exo), A549 cell-derived exosomes (+A549 exo), or A549 cell-derived exosomes plus TGF-β inhibitor SM-16 (+A549 exo+SM-16). (F) The tube formation of HUVECs following the same exposure. **p < 0.01, Student’s t test.