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. 2019 Sep 11;9:13083. doi: 10.1038/s41598-019-49581-4

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) Representative image of the frequency of regulatory B cells according to CD24 and CD27 expression in gastric cancer patients. (B) The frequency of IL-10-producing B cells in (a) CD19⁺CD24^-CD27^- B cells, (b) CD19⁺CD24^-CD27⁺ B cells, (c) CD19⁺CD24⁺CD27⁻ B cells, and (d) CD19⁺CD24^hiCD27⁺ B cells. (C) The frequency of IL-10-producing B cells was significantly higher in the CD19⁺CD24^hiCD27⁺ B cell subset than in other subsets (P < 0.0001). (D) A significant positive correlation was detected between the frequency of IL-10-producing B cells and the frequency of CD19⁺CD24^hiCD27⁺ B cells (r = 0.5309, P = 0.0021).