Table 1.

Rate constants for each step of the site specific mechanism between STV functionalized Ag NPs (M) and biotinylated Ig-G antibodies (A).

Reaction	k(M−1 s−1) Ag NPs 38 nm	k(M−1 s−1) Ag NPs 46 nm	k(M−1 s−1) Ag NPs 76 nm
(1) M + A → MA1*	(6 ± 2) × 107	(5 ± 3) × 107	(6 ± 4) × 107
(2) M + A → MA1	(4 ± 1) × 107	(3 ± 2) × 107	(5 ± 3) × 107
(3) MA1* + M → D	(5 ± 3) × 108	(7 ± 4) × 108	(4 ± 3) × 108
(4) MA1 + M → D	not allowed	not allowed	not allowed
(5) MA1* + A → MA2*	(4 ± 1) × 107	(4 ± 3) × 107	(3 ± 1) × 107
(6) MA1 + A → MA2*	(6 ± 2) × 107	(4 ± 3) × 107	(5 ± 4) × 107
(7) MA1 + A → MA2	(2 ± 1) × 107	(3 ± 1) × 107	(3 ± 2) × 107
(8) MA2 + M → D	not allowed	not allowed	not allowed
(9) MA2* + M → D	(4 ± 2) × 108	(5 ± 1) × 108	(4 ± 3) × 108
(10)MA2* + A → MA3*	(3 ± 2) × 107	(1 ± 0.5) × 107	(1 ± 0.4) × 107
(11) MA2 + A → MA3*	(5 ± 1) × 107	(1 ± 0.5) × 107	(2 ± 1) × 107
(12) MA3* + M → D	(1 ± 0.6) × 107	(1 ± 0.5) × 107	(2 ± 1) × 107

^*Species with a biotinylated IgG (IgG-Biot) in the binding reactive site of STV.