Figure 3.

Huh7.5-SEC14L2 cells transiently transfected (by electroporation) with luciferase-containing HCV (S52-replicon) constructs with the NS5b polymorphisms from patient 9 (see Table 1). Cells were treated with SOF for 72 hours and replication was determined by luciferase assay, normalized to a sample 4 hours post electroporation for each construct. As a control for SOF non-response, a replicon containing the S282T NS5b mutation was included. Panels A–E indicate the effect of the polymorphisms from patient 9 to SOF sensitivity. IC₅₀ values with 95% CIs were calculated by determining the drug concentration that affected a 50% reduction in HCV RNA and are summarized in Table 2. Mean values per drug concentration are plotted ± SEM. The above is representative of at least 3 independent experiments.