Supplementary Figure 6.

(A) Naïve Huh7.5-SEC14L2 cells were infected with Wt and DBN virus with the polymorphisms of interest at a multiplicity of infection of 0.05. Samples were taken at the indicated time points for quantification of HCV RNA by reverse-transcription quantitative polymerase chain reaction. Data presented are representative of 2 independent experiments and analyzed using a Mann-Whitney U test. Graph depicts mean ± SEM. (B, C) An equal inoculum of the Wt and mutant virus was used to infect the cells (multiplicity of infection = 0.03 median tissue culture infectious dose), the relative amounts of the competing viruses were determined by next-generation sequencing at 0, 24, 48, and 72 hours post infection and the percent of Wt (alanine at 150 or lysine at 206) is shown.