Quantification of RBC-MVs in supernatant released by RBCs during storage of 20 different packed RBCs. a The number of total MV/μl that were counted based on the FSC and SSC. Plot revealed a statistically significant increase of MV number during storage (p < 0.0001 by ANOVA). *Significant difference (p < 0.001) between pre- and post-filtration MVs, by paired t-test. #Significant change (p < 0.001) over storage period, by ANOVA. b Total count of different MVs according to the expression of Gly.A, CD42b and AnnV that represents RBC, platelet and procoagulant MVs, respectively. Results are mean + SD.