Figure 2.

RNA-Seq Analysis Highlights a Role for CTIP2 in PKA-Regulated Protein Phosphorylation in hESC-Derived MSNs

(A and B) Ingenuity pathway analysis (IPA) of MSN20 (A) and MSN40 (B) CTIP2KO DEGs shows significant enrichment of genes regulating mitochondrial function at 20 DIV, PKA and CDK5 signaling as well as dopamine-DARPP32 feedback in cAMP signaling at 40 DIV (full gene set lists for both time points are presented in Table S2).

(C) Differential expression statistics for MSN40 DEGs within the PKA gene set at fold change threshold of |1.4|, with direct CTIP2 targets highlighted in green. Genes analyzed by RT-PCR in independent control and CTIP2KO MSN samples are indicated in italic, with red asterisks marking validated differential expression.

See also Figure S3 and Tables S2 and S3.