Table 1.
Summary of dissociation constants (K d) and ΔHs derived from ITC titrations
| Proteins | Ligands | K d (μM) | ΔH (kJ mol−1) | Incompetent fractiona |
|---|---|---|---|---|
| Wild‐type | l‐methionine | 0.00020 [0.00017, 0.00034]b | −83 [−84, −81] | 0.06 [0.049–0.067]b |
| Wild‐type | d‐methionine | 3.5 [2.5, 4.6] | −55 [−57, −53] | 0.06 [0.049–0.067]b |
| N238A | l‐methionine | 78 [69, 89] | −42 [−50, −37] | 0.04 [0, 0.067]b |
| N238A | d‐methionine | 240 [190, 310] | −31 [−34, −29] | 0.31 [0.28–0.34]b |
Abbreviation: ITC, isothermal titration calorimetry.
a
Incompetent fraction is the fraction of MetQ that is apparently not able to bind to titrant.
b
About 68.3% confidence intervals determined by error‐surface projection18 are shown in square brackets.