Table 1.
Comparison of K D between ITC, SPR, or ΔTm
| a K D (μM) | |||
|---|---|---|---|
| Protein | Ligand | bITC or SPR | c Tm |
| dBASE1 | Cmpd 3 | 14 ± 1.4 | 5.9 ± 0.59 |
| Cmpd 4 | 5.4 ± 0.54 | 5 ± 0.5 | |
| Cmpd 5 | 4.8 ± 0.48 | 3.1 ± 0.31 | |
| Cmpd 6 | 3 ± 0.3 | 1.2 ± 0.12 | |
| Cmpd 7 | 2.9 ± 0.29 | 0.9 ± 0.09 | |
| Cmpd 8 | 2.9 ± 0.29 | 1.8 ± 0.18 | |
| Cmpd 9 | 1.2 ± 0.12 | 2.3 ± 0.23 | |
| Cmpd 10 | 0.6 ± 0.06 | 1.4 ± 0.14 | |
| ecGAS | PF‐06928215 | 6.7 ± 0.67 | 3.2 ± 0.32 |
| Cmpd 17 | 0.2 ± 0.02 | 0.4 ± 0.04 | |
| Cmpd 18 | 2.7 ± 0.27 | 3.1 ± 0.31 | |
| Cmpd 19 | 13 ± 1.3 | 12 ± 1.2 | |
| fNBD1 | ADP | 19 ± 1.9 | 6 ± 0.6 |
| AMPPNP | 11 ± 1.1 | 5 ± 0.5 | |
| ATP | 2.4 ± 0.24 | 1.2 ± 0.12 | |
| PhET‐ATP | 0.3 ± 0.03 | 0.3 ± 0.03 | |
a
Standard error is estimated at 10% of values.
b
Affinities were determined by ITC for BASE1 and by SPR for cGAS and NBD1.
c
Slope and intercept values for ΔTm‐derived affinities were determined without data for the proteins they were to fit.
d
Without BASE1, the global slope and intercept values are −130.3 (±5.4) and −1.9 (±0.21).
e
Without cGAS, the global slope and intercept values are −131.2 (±5.3) and −1.9 (±0.20).
f
Without NBD1, the global slope and intercept values are −129.7 (±5.2) and −2.0(±0.20).