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. 2019 Sep 10;8:e49696. doi: 10.7554/eLife.49696

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© 2019, Cayuso et al

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Figure 1. — (A–C) HCR stainings for rfng and epha4. rfng is mainly expressed in epha4-expressing cells in rhombomeres r3 and r5, with a few rfng-expressing cells in adjacent segments, for example r6 (arrowhead). (C–C’’) show a higher magnification view of an r2/r3 border. (D–F) Boundary expression of wnt1 is dorsal to rfng (D) and is co-expressed with epha4 (E, F). (A–C, E, F) are dorsal views, (D) is a lateral view, dorsal (d) top, ventral (v) bottom. Anterior to the left in all panels. Scale bar: 50 μm.