Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (A. thaliana) | ein6-1 een-1 | Roman et al. (1995) | ||
| Strain, strain background (A. thaliana) | ein6-1 | This study | Materials and methods subsection: Plant material and growth conditions | |
| Strain, strain background (A. thaliana) | een-1 | This study | Materials and methods subsection: Plant material and growth conditions | |
| Strain, strain background (A.thaliana) | ein2-45 | Beaudoin et al. (2000) | ||
| Strain, strain background (A. thaliana) | ein2-5 | Alonso et al. (1999) | ||
| Strain, strain background (A. thaliana) | ref6-1 | Noh et al. (2004) | ||
| Strain, strain background (A. thaliana) | een-2 | This study | SALKseq_129237 | Materials and methods subsection: Plant material and growth conditions |
| Strain, strain background (A. thaliana) | arp5-1 | Kandasamy et al. (2009) | ||
| Strain, strain background (A. thaliana) | ino80-1 | Fritsch et al. (2004) | ||
| Strain, strain background (A. thaliana) | ino80-8 | This study | SALKseq_041809 | Materials and methods subsection: Plant material and growth conditions |
| Strain, strain background (A. thaliana) | pie1-1 | Noh and Amasino (2003) | ||
| Strain, strainbackground (A.thaliana) | ref6-1 een-2 | This study | Materials and methods subsection: Plant material and growth conditions | |
| Strain, strain background (A. thaliana) | ref6-1 arp5-1 | This study | Materials and methods subsection: Plant material and growth conditions | |
| Strain, strain background (A. thaliana) | ref6-1 ino80-1 | This study | Materials and methods subsection: Plant material and growth conditions | |
| Strain, strain background (A. thaliana) | pie1-1 ref6-1 een-2 | This study | Materials and methods subsection: Plant material and growth conditions | |
| Strain, strain background (A. thaliana) | Col-0 HTA11:HTA11:GFP | Kumar and Wigge (2010) | ||
| Strain, strain background (A. thaliana) | Ler 35S:EIN6-FLAG | This study | Materials and methods subsection: Vectors construction and plant transformation conditions | |
| Strain, strain background (A. thaliana) | Col-0 35S:FLAG-EEN | This study | Materials and methods subsection: Vectors construction and plant transformation conditions | |
| Strain, strain background (A. thaliana) | Col-0 35S:GFP-EEN | This study | Materials and methods subsection: Vectors construction and plant transformation conditions | |
| Strain, strain background (A. thaliana) |
ein6-1 een-1
EIN6:EIN6 |
This study | Materials and methods subsection: Vectors construction and plant transformation conditions | |
| Strain, strain background (A. thaliana) |
ein6-1 een-1
EEN:EEN |
This study | Materials and methods subsection: Vectors construction and plant transformation conditions | |
| Antibody | Rabbit polyclonal anti-histone H3K27me3 | Active Motif | Cat# 39156, RRID:AB_2636821 | 5 µl Materials and methods subsection: ChIP-seq |
| Antibody | Rabbit polyclonal anti - Htz1/Histone H2A.Z | Active Motif | Cat# 39647 RRID:AB_2793289 |
10 µl Materials and methods subsection: ChIP-seq |
| Antibody | Rabbit monoclonal anti trimethyl-Histone H3 (Lys4) | Millipore Sigma | Cat#: 04–745 RRID:AB_1163444 | 4 µl Materials and methods subsection: ChIP-seq |
| Antibody | Rabbit monoclonal anti-ubiquityl-Histone H2A (Lys119) | Cell Signaling Technology | Cat#: 8240 RRID:AB_10891618 | 10 µl Materials and methods subsection: ChIP-seq |
| Antibody | Mouse monoclonal anti-FLAG M2 | Millipore Sigma | Cat#: F1804 RRID:AB_262044 | 5 µl Materials and methods subsection: ChIP-seq |
| Antibody | Mouse monoclonal anti-GFP | Millipore Sigma | Cat#:11814460001 RRID:AB_390913) | 5 µl Materials and methods subsection: ChIP-seq |
| Antibody | ChromPure Mouse IgG, whole molecule | Jackson ImmunoResearch | Cat#:015-000-003 RRID:AB_2337188 |
2 µl Materials and methods subsection: ChIP-seq |
| Antibody | Mouse monoclonal anti-HaloTag | Promega | Cat#: G9211 | 1:1000 Materials and methods subsection: Immunoblot analysis and in vitro pull-down assays |
| Antibody | Rabbit polyclonal anti-Histone H3 | Abcam | Cat#: ab1791 RRID:AB_302613) |
1:1000 Materials and methods subsection: Immunoblot analysis and in vitro pull-down assays |
| Antibody | Rabbit polyclonal anti-EIN3 | Chang et al. (2013) | 1:500 Materials and methods subsection: Immunoblot analysis and in vitro pull-down assays |
|
| Antibody | Mouse polyclonal anti-EIN2 | Qiao et al. (2012) | 1:500 Materials and methods subsection: Immunoblot analysis and in vitro pull-down assays |
|
| Chemical compound, drug | Protein G Dynabeads | Thermo Fisher Scientific | Cat. #: 10004D | 50 µl Materials and methods subsection: ChIP-seq |