Fig. 3.

Reduced L1 expression leads to elevated AMOG expression. a-c Western blot analysis of L1 and AMOG expression in U-87 MG (a), U251 (b), and SHG44 (c) cells after treatment with L1 siRNA at 5 nM. Mean values ± SEM from 4 independent experiments are presented. (*p < 0.05, **p < 0.01, *** p < 0.001 vs. Control siRNA, independent Student’s t-test). d-f Representative images of double immunofluorescence staining of L1 and AMOG in response to L1 siRNA at 5 nM in U-87 MG (d), U251 (e) and SHG44 (f) cells. Scale bars = 50 μm