Fig. 1.

ZIKV causes a lethal disease in WT, Rag1^−/−, and Ifnar1^−/− mice following intracranial infection. WT (n = 13, solid lines), Ifnar1^−/− (n = 7, dashed lines), and Rag1^−/− (n = 8, dotted lines) mice were infected i.c. with ZIKV MR766. Sham-injected mice (n = 3 per genotype) received virus diluent only. Survival (a), weight loss (b), and clinical scores (c) were determined daily. Mean ± SEM are shown, and additional horizontal dotted lines indicate humane endpoint criteria as approved by the University of Sydney Animal Ethics Committee. For a, *p < 0.05 compared with WT, ^p < 0.05 compared with Ifnar1^−/−, and n.s. = not significant as determined by Log-rank test. For b, *p < 0.05 when compared to the corresponding time point in WT mice and ^p < 0.05 when compared to the corresponding time point in Rag1^−/− mice, as determined by two-way ANOVA. One of two representative experiments is shown