Table 2.

Corneal features after decellularization by new and traditional methods.

		New method	Traditional methods
		Method A	Method B	Method C
Corneal transparency		Declined slightly	Declined seriously
Alignment of the stromal fibrils		Regular	Irregular
Residual nuclei		No obvious	Obvious
Thickening rate (%)		204.90 ± 3.95	531.90 ± 7.93	410.30 ± 3.69
			p < 0.0001 (compared with method A)
Moisture content (%)		91.80 ± 0.37	94.42 ± 0.25	94.04 ± 0.23
			p < 0.01 (compared with method A)
DNA clearance rate		About 98.67%	About 61.77%	About 91.65%
GAG content (ng/mg)		No significant changes (1.65 ± 0.05, p > 0.05)	Significant decreased
			(1.19 ± 0.11, p < 0.05)	(1.25 ± 0.08, p < 0.05)
Mechanical properties	Young’s modulus	Significant increase	Significant decline
	Tensile stress	Great in a short time	Weak in a long time

GAG: glycosaminoglycan.