(A) Representative HPLC-DAD chromatogram of
a solution containing
100 μg mL–1 of each of LSD, F: 25F-NBOMe (2a), Cl: 25C-NBOMe (2b), Br: 25B-NBOMe (2c), and I: 25I-NBOMe (2d). (B) Representative
HPLC-AD amperogram of a solution containing 100 μg mL–1 of each of LSD, F: 25F-NBOMe (2a), Cl: 25C-NBOMe (2b), Br: 25B-NBOMe (2c), and I: 25I-NBOMe (2d). Experimental parameters include ACE C18-AR column (150
× 4.6 mm2 i.d., particle size: 5 μm), mobile
phase: [5 mM ammonium formate + 100 mM KCl (pH 7.0): acetonitrile
70:30% (v/v)], flow rate: 2.5 mL min–1, column temperature:
60 °C, detector wavelength (UV): 205 nm, and potential: +1.0
V.