Table 3. Summary of the Optimized Experimental Parameters Chosen for the Separation and Quantitation of NBOMe Derivatives.

studied experimental parameter	optimized parameter
analytical column	ACE 5 C18-AR column (150 × 4.6 mm2 i.d., particle size: 5 μm)
mobile phase	isocratic (5 mM ammonium formate + 100 mM KCl:acetonitrile 70:30% v/v)
ionic strength of ammonium formate buffer	5 mM
pH of the aqueous phase	pH 7
linear velocity of the mobile phase	2.5 mL min–1
column temperature	60 °C
applied potential for amperometric detection (AD)	+1.0 V
detection wavelength λ for photodiode array detection (DAD)	205 nm