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. 2019 Aug 27;4(11):14626–14632. doi: 10.1021/acsomega.9b02107

Figure 1.

Figure 1

(A) Diagram of the pMoT series plasmid system. PslpA is the 177-bp Tth SlpA gene promoter. Indicated with arrows of different colors are the component modules; also shown are the restriction sites connecting the modules. Tth, T. thermophilus; Ec, E. coli. See Table S4 for a list of the module parts. (B) Summary of pMoT component parts. Pnqo, promoter for the nqo gene; P16S, promoter for the Tth 16S RNA; thermosensors, translation control structures described in this work; 3Knqo72, fusion of promoter nqo72 plus promoter 3K; PslpA, promoter for S-layer A gene; Pnar, nitrate reductase promoter; sIFP, superfolder citrine GFP; sGFP, superfolder GFP; β-gal, β-galactosidase; CrtB, β-carotene synthesis gene; AP, alkaline phosphatase; PFEI, esterase I variety 34 from P. fluorescens. See the Supporting Information for references. Length of the DNA fragments, in Kbs, is indicated between parentheses. (C) Sequence of the different multiple cloning sites indicating the unique restriction sites. Pnqo, promoter of the gene nqo; RBS, ribosome binding site. Linkers 1, 2, and 3 are designed to clone fragments and translate them in the three different frames; translation in the corresponding frame is shown.