FIGURE 2:

Vezatin associates with AChR subunits. cDNAs encoding Flag-vezatin and CD4-AChR subunit MA loop chimeras were cotransfected into HEK 293 cells. CD4-AChR subunit MA loop chimeras were immunoprecipitated with antibodies to CD4, and Western blots were probed with antibodies to Flag or CD4. The four CD4-AChR subunit chimeras were expressed at different levels, so the amount of coprecipitated Flag-vezatin was normalized to the expression level of each CD4-AChR subunit MA loop chimera (n = 3). Thirty-fold more Flag-vezatin associated with the CD4-β chimeras than CD4 alone (n = 3). Vezatin also associated with the AChR δ, AChR ε, and AChR α subunit chimeras. Error bars indicate SEM, and p values were calculated using an unpaired t test (*, p < 0.05; **, p < 0.005; ***, p < 0.0005).