FIGURE 3:

Vezatin is enriched at the postsynaptic membrane at neuromuscular synapses. (A) Cross-sections of innervated adult mouse gastrocnemius muscles were stained with Alexa 594–conjugated α-BGT to label AChRs, antibodies to synapsin to label nerve terminals, and antibodies to vezatin or MuSK. Vezatin is enriched at synaptic sites (arrows). (B) Single muscle fibers from adult TA muscles were dissociated and stained with Alexa 594–conjugated α-BGT, antibodies to synapsin, and antibodies to vezatin or rapsyn. Most dissociated myofibers lacked nerve terminals. Vezatin remained enriched at synaptic sites in the absence of nerve terminals. Scale bar = 20 μm.