Skip to main content
. Author manuscript; available in PMC: 2019 Sep 12.
Published in final edited form as: Mol Pharm. 2019 May 13;16(6):2354–2363. doi: 10.1021/acs.molpharmaceut.8b01274

Figure 1.

Figure 1.

Fluorescence values of surface versus internalized proteoliposomes determined in pre-fixed CEACAM1 HeLa cells. CEACAM1 HeLa cells were dissociated and fixed with 4% PFA prior to incubation with Opa60 proteoliposomes (yellow). (A) Surface and internalization masks were used to determine adhered versus internalized liposome fluorescence. Internalization masks were defined as being a set percent of the full brightfield mask (75–95%) while surface masks were defined as being fluorescence outside of each internalization mask. White scale bar is set to 7 μm. (B) When used to quantify internal and surface proteoliposome fluorescence in pre-fixed cells, which are not expected to internalize liposomes following binding, high surface and low internal (6.2% of total) liposome fluorescence was measured with an Adaptive Erode mask set to 75% of full brightfield. Error bars represent 95% C.I.