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Opaless liposome size inversely correlates with Opa-independent uptake. Control (white), CEACAM1 (black), and CEACAM3 (grey) HeLa cells were incubated with 0.2 mM [phospholipid] of non-proteinaceous liposomes that ranged in diameter from approximately 30 to 400 nm. The highest Opa-independent uptake occurred in cells exposed to 30 nm liposomes, with liposome uptake decreasing as liposome size increases. Error bars represent 95% C.I.
