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. Author manuscript; available in PMC: 2019 Sep 12.
Published in final edited form as: Mol Pharm. 2019 May 13;16(6):2354–2363. doi: 10.1021/acs.molpharmaceut.8b01274

Figure 5.

Figure 5.

Opa60 promotes proteoliposome uptake into HeLa cells. HeLa cells were pulsed for 15 min with proteoliposomes, then chased in fresh medium without liposomes for 0, 1, 2, or 3 hrs. Liposome internalization efficiency (internal/surface fluorescence) was determined for control (A), CEACAM1 (B), and CEACAM3 (C) HeLa cells following exposure to Opa60 (black) and Opa(HV−) (red) proteoliposomes. Opa60 enhances proteoliposome uptake into HeLa cells compared to Opa(HV−), shown as higher Internal/Surface values. The increase or decrease in proteoliposome internalization efficiency from Opa(HV−) to Opa60 is shown as a percent of baseline Opa(HV−) proteoliposome efficiency on each graph. These values were plotted in (D), showing that as time increases, efficiency of Opa60 internalization when compared to Opa(HV−) internalization increases only within the context of CEACAM1 HeLa cells. Error bars represent 95% C.I.